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KMID : 0861020070220020097
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Korea Journal of Herbology
2007 Volume.22 No. 2 p.97 ~ p.102
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Analysis of Marker Substances in Samul-tang by HPLC-MS/MS
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Yu Young-Beob
Kim Mi-Jung
Hwang Dae-Sun
Ha Hye-Kyeong
Ma Jin-Yeul
Shin Hyeun-Kyoo
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Abstract
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Objectives : This study presents a high performance liquid chromatography - electrospray ionization-mass spectrometer (HPLC-MS/MS) methods for the quantitative and qualitative analysis of various active components in Samul-tang, which is composed of four crude herbs.
Methods : HPLC-ESI-MS/MS for the determinations of paeoniflorin and 5-HMF (5-hydroxymethyl 2-furaldehyde) in the Samul-tang, the separation method was performed on an COSMOS1L 5C18-AR-Il (2.0 X 150 mm I.D.) column by gradient elution with 0.1% acetic acid and 5% CH3CN in water (A)-0.1% acetic acid and 5% H20 in CH3CN (B) as the mobile phase at a flow-rate of 300 {mu}L/min with detection at quadrupole mass spectrometer. The all marker substances were always detected as the base peaks in the positive ion mode.
Results : The paeoniflorin of Paeoniae Radix in Samul-tang showed a strong base peak [M+H2O]+ in the positive detection mode to give the following as; paeoniflorin (498.109 [M+H2O]+, 479.8 [M]+, 301 [M-glucose]+, 179.3 [glucose]+). Based on the HPLC retnetion time and MS of standard compounds confirmed the identity of active compounds in Rehmanniae Radix Preparata as follows; 5-HMF (127.0[M+H]+, 109.3 [M-OH]+) in the positive ion mode.
Conclusion : According to the above results, HPLC-ESI-MS method permits assignment of tentative structures such as paeoniflorin and 5-HMF in the Samul-tang.
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KEYWORD
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Samul-tang, HPLC-MS/MS, paeoniflorin, 5-HMF
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